涂层和未涂层正畸弓丝上的细菌定植：一项前瞻性临床试验。
Bacterial colonization on coated and uncoated orthodontic wires: A prospective clinical trial.
作者信息
Raji Seyed Hamid, Shojaei Hasan, Ghorani Parinaz Saeidi, Rafiei Elahe
机构信息
Torabinejad Research Center and Department of Orthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Microbiology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Dent Res J (Isfahan). 2014 Nov;11(6):680-3.
BACKGROUND
The advantages of coated orthodontic wires such as esthetic and their effects on reduced friction, corrosion and allergic reaction and the significant consequences of plaque accumulation on oral health encouraged us to assess bacterial colonization on these wires.
MATERIALS AND METHODS
A total of 18 (9 upper and 9 lower) epoxy resin coated 16 × 22 nickel-titanium wires (Spectra, GAC, USA) and 18 (9 upper and 9 lower) non-coated 16 × 22 nickel-titanium wires (Sentalloy, GAC, USA) with isolated packages were selected and sterilized before application. The samples were divided randomly between upper and lower arches in 18 patients and hence that every patient received one coated and one uncoated wire at the same time. Samples were removed and cut in equal lengths after 3 weeks and placed in phosphate buffered saline buffer. After separation of bacteria in trypsin and ethylenediaminetetraacetic acid solution, the diluted solution was cultured in blood agar and bacterial colony forming units were counted. Finally, the data was analyzed using the paired t-test and the significance was set at 0.05.
RESULTS
Mean of bacterial colonization on uncoated wires was more than that of coated wires (P < 0.001).
CONCLUSION
Bacterial plaque accumulation on epoxy resin coated nickel-titanium orthodontic wires is significantly lower than uncoated nickel-titanium wires.
背景
正畸带涂层弓丝具有美观等优点，且对减少摩擦、腐蚀和过敏反应有作用，同时牙菌斑堆积对口腔健康有重大影响，这些促使我们评估这些弓丝上的细菌定植情况。
材料与方法
共选取18根（上、下颌各9根）环氧涂层16×22镍钛弓丝（Spectra，美国GAC公司）和18根（上、下颌各9根）未涂层16×22镍钛弓丝（Sentalloy，美国GAC公司），包装独立，使用前进行灭菌。将样本随机分配到18例患者的上下牙弓，因此每位患者同时接受一根涂层弓丝和一根未涂层弓丝。3周后取出样本并等长切割，置于磷酸盐缓冲盐溶液中。在胰蛋白酶和乙二胺四乙酸溶液中分离细菌后，将稀释溶液接种于血琼脂培养基中，计数细菌菌落形成单位。最后，采用配对t检验分析数据，显著性水平设定为0.05。
结果
未涂层弓丝上的细菌定植平均数高于涂层弓丝（P < 0.001）。
结论
环氧涂层镍钛正畸弓丝上的牙菌斑堆积明显低于未涂层镍钛弓丝。
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