TiO 涂层不锈钢正畸丝对细菌的影响：一项临床研究。
Effects of TiO-Coated Stainless Steel Orthodontic Wires on Bacteria: A Clinical Study.
机构信息
Orthodontic Department, Dental Faculty and Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Int J Nanomedicine. 2020 Nov 10;15:8759-8766. doi: 10.2147/IJN.S258440. eCollection 2020.
INTRODUCTION
The aim of this study was to clinically evaluate adhesion on titanium dioxide-coated stainless steel orthodontic wires to decrease white-spot formation.
METHODS
In this study, four groups of 17 patients each (n=68) aged 12-25 years participated. A titanium dioxide coating layer was deposited on 0.4572 mm stainless steel orthodontic wires using physical vapor deposition. The coated wires were randomly assigned to one jaw, and the opposite jaw received an uncoated wire as control. Patients were divided into groups according to the duration that wires were in their mouths: A) 1 week, B) 2 weeks, C) 3 weeks, and D) 4 weeks. Block randomization was used to assign patients to each group. At the end of the experiment, 20 mm of each wire (canine-to-canine area) was cut and cultured in -specific medium. The culture plates were placed in an incubator containing 5% CO for 72 hours at 37°C, and then colonies were counted. MTT was used to test the biocompatibility of the coated and uncoated wires. To evaluate the stability of the coated titanium dioxide layer on the wires, titanium concentration on the saliva was determined using inductively coupled plasma mass spectroscopy.
RESULTS
The Kruskal-Wallis test showed that there was no significant difference in colony counts among the coated wires during 1-4 weeks (<0.48). In the uncoated-wire groups, colonys count at week 1 were higher than weeks 24 -(<0.022). Wilcoxon's test showed that the number of colonies was significantly different in groups A and C, but there was no significant difference in groups B or D. MTT-assay results showed that there was not a significant difference between cell viability in the coated-wire group and the control. The Kruskal-Wallis test showed that there was no significant difference in titanium concentration in the studied groups (<0.834).
CONCLUSION
Application of titanium dioxide coating is effective in reducing bacterial adhesion at wire insertion.
简介
本研究旨在临床评估涂覆二氧化钛的不锈钢正畸丝的黏附性，以减少白斑的形成。
方法
本研究纳入了 4 组各 17 例（n=68）年龄在 12-25 岁的患者。采用物理气相沉积法在 0.4572 毫米不锈钢正畸丝上沉积一层二氧化钛涂层。将涂覆的金属丝随机分配到一侧颌骨，对侧颌骨使用未涂覆的金属丝作为对照。根据金属丝在口腔中的时间将患者分为 4 组：A）1 周，B）2 周，C）3 周，D）4 周。采用区组随机化方法将患者分配到每组。实验结束时，从每根金属丝（尖牙到尖牙区域）的 20 毫米处剪下并在特定培养基中培养。将培养板置于含有 5%CO 的孵育箱中，在 37°C 下孵育 72 小时，然后计数菌落。MTT 用于测试涂覆和未涂覆金属丝的生物相容性。为了评估金属丝上涂覆的二氧化钛层的稳定性，使用电感耦合等离子体质谱法测定唾液中的钛浓度。
结果
Kruskal-Wallis 检验显示，涂覆金属丝在 1-4 周内的菌落计数无显著差异（<0.48）。在未涂覆金属丝组中，第 1 周的菌落计数高于第 2-4 周（<0.022）。Wilcoxon 检验显示，A 组和 C 组的菌落数量有显著差异，但 B 组和 D 组无显著差异。MTT 检测结果显示，涂覆金属丝组与对照组的细胞活力无显著差异。Kruskal-Wallis 检验显示，研究组之间的钛浓度无显著差异（<0.834）。
结论
涂覆二氧化钛可有效减少金属丝插入时的细菌黏附。
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