Bordoni A, Biagi P L, Parenti Castelli G, Hrelia S, Rossi C A, Lercker G, Izpisua J C, Barber T, Cabo J, Lenaz G
Dipartimento di Biochimica, Universita' di Bologna, Italy.
Membr Biochem. 1989;8(1):11-8. doi: 10.3109/09687688909025822.
Feeding rats a hyperlipidic diet in which animals were offered daily a variety of high-energy food resulted in a significant increase of serum free fatty acids and a decrease of phospholipids with respect to controls. On the contrary, there were no significant differences in erythrocyte membrane total lipid composition between the two groups. Erythrocyte membranes showed a significant decrease in saturated fatty acid content and a significant increase in (n-6) polyunsaturated fatty acid content; (n-3) polyunsaturated fatty acids significantly decreased. Membrane fluidity, investigated by fluorescence polarization of diphenylhexatriene, significantly increased in the erythrocyte membranes of the experimental group. These results seem compatible with the decreased saturated/unsaturated fatty acid ratio. A significant decrease of (Na+-K+)ATPase activity occurred in erythrocyte membranes of the experimental group rats with respect to the controls.
给大鼠喂食高脂饮食，即每天给动物提供各种高能食物，结果血清游离脂肪酸显著增加，磷脂相对于对照组减少。相反，两组之间红细胞膜总脂质组成没有显著差异。红细胞膜显示饱和脂肪酸含量显著降低，（n-6）多不饱和脂肪酸含量显著增加；（n-3）多不饱和脂肪酸显著减少。通过二苯基己三烯荧光偏振研究的膜流动性在实验组红细胞膜中显著增加。这些结果似乎与饱和/不饱和脂肪酸比例降低相一致。实验组大鼠红细胞膜中（Na+-K+）ATP酶活性相对于对照组显著降低。
