Isberg Vignir, de Graaf Chris, Bortolato Andrea, Cherezov Vadim, Katritch Vsevolod, Marshall Fiona H, Mordalski Stefan, Pin Jean-Philippe, Stevens Raymond C, Vriend Gerrit, Gloriam David E
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Division of Medicinal Chemistry, Faculty of Sciences, Amsterdam Institute for Molecules, Medicines and Systems, VU University Amsterdam, The Netherlands.
Trends Pharmacol Sci. 2015 Jan;36(1):22-31. doi: 10.1016/j.tips.2014.11.001. Epub 2014 Dec 22.
Generic residue numbers facilitate comparisons of, for example, mutational effects, ligand interactions, and structural motifs. The numbering scheme by Ballesteros and Weinstein for residues within the class A GPCRs (G protein-coupled receptors) has more than 1100 citations, and the recent crystal structures for classes B, C, and F now call for a community consensus in residue numbering within and across these classes. Furthermore, the structural era has uncovered helix bulges and constrictions that offset the generic residue numbers. The use of generic residue numbers depends on convenient access by pharmacologists, chemists, and structural biologists. We review the generic residue numbering schemes for each GPCR class, as well as a complementary structure-based scheme, and provide illustrative examples and GPCR database (GPCRDB) web tools to number any receptor sequence or structure.
通用残基编号便于进行例如突变效应、配体相互作用和结构基序等方面的比较。巴列斯特罗斯和温斯坦针对A类G蛋白偶联受体（GPCRs）内的残基所采用的编号方案已有超过1100次引用，而最近B、C和F类GPCRs的晶体结构现在要求在这些类别内部和类别之间就残基编号达成社区共识。此外，结构时代已经发现了螺旋凸起和收缩，这使得通用残基编号出现了偏差。通用残基编号的使用依赖于药理学家、化学家和结构生物学家能够方便地获取。我们回顾了每个GPCR类别以及一个基于结构的补充编号方案的通用残基编号方案，并提供了说明性示例和GPCR数据库（GPCRDB）网络工具，用于对任何受体序列或结构进行编号。
