Yang Yajun, Wu Zhihong, Xu Hongxing, Zheng Xusong, Tian Junce, Lu Zhongxian
a State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology , Zhejiang Academy of Agricultural Sciences , Hangzhou , China and.
b School of Life and Environment Science, Hangzhou Normal University , Hangzhou , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):3991-3994. doi: 10.3109/19401736.2014.989519. Epub 2014 Dec 26.
Rice leaffolders Cnaphalocrocis medinalis and Marasmia patnalis (Lepidoptera: Pyralidae) are important rice pests and morphologically related with same damage methods. The control region of mitochondrial DNA (mtDNA) in two rice leaffolders C. medinalis and M. patnalis were sequenced and analyzed. The control regions of the two rice leaffolders are located between 12S ribosomal RNA and tRNA, and exhibit conserved structural elements. We identified the poly T stretches, (TA)n block, and stem-loop structure. There are no long tandem repeats found in mtDNA control region in the two rice leaffolders studied. Nevertheless, we did not find the GA-rich block in mtDNA control regions of the two rice leaffolders. The molecular-based phylogenies support the traditional morphologically based view of relationships of Pyralidae within the Ditrysia. The addition of the C. medinalis and M. patnalis mtDNA control regions to the literature promotes the understanding of the molecular evolution of Pyralidae in Ditrysia.
稻纵卷叶螟和显纹纵卷叶螟（鳞翅目：螟蛾科）是重要的水稻害虫，形态相似且危害方式相同。对两种稻纵卷叶螟，即稻纵卷叶螟和显纹纵卷叶螟线粒体DNA（mtDNA）的控制区进行了测序和分析。两种稻纵卷叶螟的控制区位于12S核糖体RNA和tRNA之间，并呈现出保守的结构元件。我们鉴定出了多聚T序列、（TA）n块和茎环结构。在所研究的两种稻纵卷叶螟的mtDNA控制区中未发现长串联重复序列。然而，我们在两种稻纵卷叶螟的mtDNA控制区中未发现富含GA的区域。基于分子的系统发育支持了传统的基于形态学的螟蛾科在双孔亚目内的亲缘关系观点。将稻纵卷叶螟和显纹纵卷叶螟的mtDNA控制区补充到文献中，有助于增进对双孔亚目螟蛾科分子进化的理解。
