Tripathy Satyajit, Chattopadhyay Sourav, Dash Sandeep Kumar, Chowdhuri Angshuman Ray, Das Sabyasachi, Sahu Sumanta Kumar, Majumdar Subrata, Roy Somenath
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721102, West Bengal, India.
Department of Applied Chemistry, Indian School of Mines, Dhanbad 826004, Jharkhand, India.
Int J Biol Macromol. 2015 Mar;74:585-600. doi: 10.1016/j.ijbiomac.2014.12.016. Epub 2014 Dec 24.
Chitosan has impelled continuous motion by its unique physicochemical and biological characteristics. In our study, chitosan-tripolyphosphate (CS-TPP) particles was conjugated with an undervalued antimalarial drug, chloroquine to find out the proficiency against ROS mediated caspase activation and apoptosis in liver during Plasmodium berghei NK65 infection. The transmission electron microscopic image illustrated the size range of particle was less than 50 nm and the particle showed the blood compatibility. ROS generation, mitochondrial membrane potential, anti apoptotic and pro apoptotic protein level of CS-TPP conjugated chloroquine treated group revealed that CS-TPP conjugation amplified the protective capability of chloroquine. FACS study by annexin v-FITC and PI staining reveals chloroquine treatment reduces significantly (P<0.05) the apoptotic cells by 25.31%; whereas chitosan-tripolyphosphate conjugated nanochloroquine decreases by 61.56% apoptotic cell against P. berghei induced liver apoptosis. This study suggests that proficiency of conventional antimalarial drug may escalate by delivery with chitosan nanoparticles to portray defense possessions against malarial damage.
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