Erdö S L, Wolff J R
Department of Anatomy, Georg August University, Göttingen (F.R.G.).
Neurosci Lett. 1989 Sep 25;104(1-2):161-6. doi: 10.1016/0304-3940(89)90348-0.
The postnatal changes in the specific binding of [3H]kainate and [3H]AMPA (RS-alpha-amino-3-hydroxy-5-methyl-4-isoxasolopropionic acid, an agonist of quisqualate receptors) were studied in cerebral cortex of rats, aged 2-360 days. The binding of the two ligands was assayed in whole-tissue homogenates. Similar developmental time courses were found for kainate and AMPA binding, characterized by high perinatal levels, a further increase during the first few days after birth, an early maximum value around the age of one week, and a gradual decrease to adult values which were attained at an age of 3-4 weeks. As revealed by Scatchard analysis, the transient elevation of ligand binding was derived from an increased density of binding sites, which, in the case of AMPA, was accompanied also by an increase in binding affinity. The results indicate that, in the immature cerebral cortex, kainate and quisqualate receptors may play a role other than in synaptic transmission.
研究了2至360日龄大鼠大脑皮层中[3H]海人酸和[3H]AMPA（RS-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸，一种使君子酸受体激动剂）特异性结合的产后变化。在全组织匀浆中测定了两种配体的结合情况。海人酸和AMPA结合呈现相似的发育时间进程，其特点是围产期水平较高，出生后最初几天进一步升高，在约一周龄时出现早期最大值，随后逐渐下降至3至4周龄时达到的成年值。Scatchard分析表明，配体结合的短暂升高源于结合位点密度的增加，就AMPA而言，还伴随着结合亲和力的增加。结果表明，在未成熟的大脑皮层中，海人酸和使君子酸受体可能在突触传递之外发挥作用。
