Massicotte G, Baudry M
Program in Neural, Informational, and Behavioral Sciences, University of Southern California, Los Angeles 90089.
Neurosci Lett. 1990 Oct 16;118(2):245-8. doi: 10.1016/0304-3940(90)90638-p.
The expression of long-term potentiation (LTP) in area CA1 of hippocampus has been proposed to result from an increased sensitivity of the AMPA/quisqualate receptors. We have investigated the binding properties of excitatory amino acid receptors in phospholipase A2 (PLA2)-treated rat brain membranes. PLA2 from bee venom produced a significant increase in the binding of [3H]-AMPA ([3H]-amino-3-hydroxy-5-methylisoxazole-4- propionate), a ligand for the AMPA/quisqualate receptor. Analysis of the saturation kinetics revealed that PLA2 treatment increased the affinity of the AMPA/quisqualate receptor without changing the maximum number of sites. In contrast, PLA2 treatment did not detectably modify the binding of [3H]-kainate to the kainate receptor and of [3H]-glutamate and [3H]-glycine to the NMDA (N-methyl-D-aspartate) receptor complex. These finding suggest that phospholipase A2 may regulate the AMPA/quisqualate receptor and could play an important role in the development of LTP.
海马体CA1区的长时程增强（LTP）表达被认为是由AMPA/quisqualate受体敏感性增加所致。我们研究了磷脂酶A2（PLA2）处理的大鼠脑膜中兴奋性氨基酸受体的结合特性。蜂毒中的PLA2使[3H]-AMPA（[3H]-氨基-3-羟基-5-甲基异恶唑-4-丙酸）的结合显著增加，[3H]-AMPA是AMPA/quisqualate受体的配体。饱和动力学分析表明，PLA2处理增加了AMPA/quisqualate受体的亲和力，而不改变位点的最大数量。相反，PLA2处理未检测到对[3H]-海人藻酸与海人藻酸受体的结合以及[3H]-谷氨酸和[3H]-甘氨酸与NMDA（N-甲基-D-天冬氨酸）受体复合物的结合有明显影响。这些发现表明，磷脂酶A2可能调节AMPA/quisqualate受体，并可能在LTP的发展中起重要作用。
