Li Xingnan, Franke Adrian A
University of Hawai'i Cancer Center, 701 Ilalo Street, Honolulu, HI 96813, United States.
University of Hawai'i Cancer Center, 701 Ilalo Street, Honolulu, HI 96813, United States.
Steroids. 2015 Jul;99(Pt A):84-90. doi: 10.1016/j.steroids.2014.12.005. Epub 2014 Dec 25.
Estrogen metabolites are important biomarkers to evaluate cancer risks and metabolic diseases. Due to their low physiological levels, a sensitive and accurate method is required, especially for the quantitation of unconjugated forms of endogenous steroids and their metabolites in humans. Here, we evaluated various derivatives of estrogens for improved analysis by orbitrap LC/MS in human serum samples. A new chemical derivatization reagent was applied modifying phenolic steroids to form 1-methylimidazole-2-sulfonyl adducts. The method significantly improves the sensitivity 2-100 fold by full scan MS and targeted selected ion monitoring MS over other derivatization methods including, dansyl, picolinoyl, and pyridine-3-sulfonyl products.
雌激素代谢物是评估癌症风险和代谢疾病的重要生物标志物。由于其生理水平较低，需要一种灵敏且准确的方法，特别是用于定量测定人体内未结合形式的内源性类固醇及其代谢物。在此，我们评估了雌激素的各种衍生物，以通过轨道阱液相色谱/质谱法改进对人血清样本的分析。应用了一种新的化学衍生化试剂，将酚类类固醇修饰形成1-甲基咪唑-2-磺酰加合物。与包括丹磺酰、吡啶甲酰和吡啶-3-磺酰产物在内的其他衍生化方法相比，该方法通过全扫描质谱和靶向选择离子监测质谱，灵敏度显著提高了2至100倍。
