Wu Wei, Xu Yuzhu, He Xinliang, Lu Yan, Guo Yali, Yin Zhuoran, Xie Jungang, Zhao Jianping
Department of Respiratory and crit care , Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Asian Pac J Allergy Immunol. 2014 Dec;32(4):337-44. doi: 10.12932/AP0450.32.4.2014.
Although it is recognized that IL-33 plays a key role in the onset of asthma, it is currently unclear whether IL-33 acts on any other target cells besides mast cells and Th2 cells in asthma. We investigated that whether airway smooth muscle cells (ASMCs) could contribute to asthma via stimulation with IL-33.
To create a mouse model of acute asthma, murine ASMCs were isolated and cultured in vitro with IL-33. The ASMCs were divided into two groups, ASMCs from normal mice and ASMCs from ovalbumin-sensitized mice. The release of mouse KC was analyzed by PCR and ELISA. Immunocytochemical Staining of murine ASMCs for ST2 and IL-1RAcP was performed.
IL-33 promoted KC expression, both in terms of mRNA and protien levels, in ASMCs from ovalbumin-sensitized mice. ST2 and IL-1RAcP were expressed in the membrane of ASMCs in ovalbumin-sensitized mice.
IL-33 may contribute to the inflammation in the airways by acting on airway smooth muscle cells. IL-33 and ST2 may play important roles in allergic bronchial asthma.
尽管人们认识到白细胞介素-33（IL-33）在哮喘发病中起关键作用，但目前尚不清楚在哮喘中，IL-33除作用于肥大细胞和辅助性T2（Th2）细胞外，是否还作用于其他靶细胞。我们研究了气道平滑肌细胞（ASMCs）是否会通过IL-33刺激而导致哮喘。
为建立急性哮喘小鼠模型，分离小鼠ASMCs并在体外与IL-33一起培养。将ASMCs分为两组，正常小鼠的ASMCs和卵清蛋白致敏小鼠的ASMCs。通过聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）分析小鼠趋化因子（KC） 的释放。对小鼠ASMCs进行ST2和白细胞介素-1受体拮抗剂（IL-1RAcP）的免疫细胞化学染色。
IL-33促进了卵清蛋白致敏小鼠的ASMCs中KC的表达，无论是在mRNA水平还是蛋白水平。ST2和IL-1RAcP在卵清蛋白致敏小鼠的ASMCs膜中表达。
IL-33可能通过作用于气道平滑肌细胞而导致气道炎症。IL-33和ST2可能在过敏性支气管哮喘中起重要作用。
