Tarragona Evelina L, Cicuttin Gabriel L, Mangold Atilio J, Mastropaolo Mariano, Nazarena De Salvo M, Nava Santiago
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300 Rafaela, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, RP Kreder 2805, S3080HOF, Esperanza, Santa Fe, Argentina.
Instituto de Zoonosis Luis Pasteur-Ministerio de Salud-Gobierno de la Ciudad Autónoma de Buenos Aires, Av Díaz Vélez 4821 C1405DCD CABA, Argentina.
Ticks Tick Borne Dis. 2015 Mar;6(2):173-7. doi: 10.1016/j.ttbdis.2014.11.010. Epub 2014 Dec 18.
The present study was performed to evaluate the Rickettsia infection in Amblyomma tonelliae ticks from Argentina. All ticks were subjected to DNA extraction and tested by a battery of PCRs to amplify fragments of four rickettsial genes, 23S-5S, gltA, ompA and htrA. Two ticks were positive. The Rickettsia detected in one tick represents a new lineage which is named Rickettsia sp. strain El Tunal. This new strain belongs to the canadensis group because it is closely related to Rickettsia monteiroi, Rickettsia canadensis and Candidatus "Rickettsia tarasevichiae". They clustered together on a high supported clade with both gltA and htrA genes. The other positive tick was infected with Candidatus "Rickettsia amblyommii". The results presented in this study constitute the first records of Rickettsia infection in A. tonelliae ticks. However, the medical relevance of these findings should be considered cautiously because the pathogenicity of Rickettsia sp. strain El Tunal and Candidatus "R. amblyommii" remains undetermined.
本研究旨在评估来自阿根廷的托氏钝眼蜱中的立克次体感染情况。所有蜱均进行DNA提取，并通过一系列聚合酶链反应（PCR）检测，以扩增四个立克次体基因（23S - 5S、gltA、ompA和htrA）的片段。有两只蜱呈阳性。在一只蜱中检测到的立克次体代表一个新的谱系，命名为立克次体属菌株埃尔图纳尔。这个新菌株属于加拿大立克次体组，因为它与蒙氏立克次体、加拿大立克次体和“塔拉舍维奇立克次体”密切相关。它们在gltA和htrA基因的高支持分支上聚集在一起。另一只阳性蜱感染了“安氏立克次体”。本研究的结果构成了托氏钝眼蜱中立克次体感染的首次记录。然而，这些发现的医学相关性应谨慎考虑，因为立克次体属菌株埃尔图纳尔和“安氏立克次体”的致病性仍未确定。
