Lewis C M, Bosman C A, Fries P
Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt, Germany; Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, 6525 EN Nijmegen, Netherlands.
Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, 6525 EN Nijmegen, Netherlands; Cognitive and Systems Neuroscience Group, Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, 1098 XH Amsterdam, Netherlands.
Curr Opin Neurobiol. 2015 Jun;32:68-77. doi: 10.1016/j.conb.2014.12.007. Epub 2014 Dec 24.
Brain activity reveals exquisite coordination across spatial scales, from local microcircuits to brain-wide networks. Understanding how the brain represents, transforms and communicates information requires simultaneous recordings from distributed nodes of whole brain networks with single-cell resolution. Realizing multi-site recordings from communicating populations is hampered by the need to isolate clusters of interacting cells, often on a day-to-day basis. Chronic implantation of multi-electrode arrays allows long-term tracking of activity. Lithography on thin films provides a means to produce arrays of variable resolution, a high degree of flexibility, and minimal tissue displacement. Sequential application of surface arrays to monitor activity across brain-wide networks and subsequent implantation of laminar arrays to target specific populations enables continual refinement of spatial scale while maintaining coverage.
大脑活动揭示了从局部微电路到全脑网络的跨空间尺度的精确协调。理解大脑如何表征、转换和传递信息需要以单细胞分辨率从全脑网络的分布式节点进行同步记录。实现对相互作用群体的多部位记录受到日常隔离相互作用细胞簇的需求的阻碍。多电极阵列的长期植入允许对活动进行长期跟踪。薄膜光刻提供了一种生产可变分辨率阵列、高度灵活性和最小组织移位的方法。依次应用表面阵列来监测全脑网络的活动，随后植入层流阵列以靶向特定群体，这使得在保持覆盖范围的同时能够不断细化空间尺度。
