Wiener Dominique J, Doherr Marcus G, Müller Eliane J, Suter Maja M, Welle Monika M
Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, PO Box 8466, 3001, Bern, Switzerland.
Dermfocus, Vetsuisse Faculty, Inselspital, Bern University Hospital, Freiburgstrasse 14, 3010, Bern, Switzerland.
Vet Dermatol. 2015 Jun;26(3):198-201, e41-2. doi: 10.1111/vde.12192. Epub 2014 Dec 29.
Follicular stem cells and their progeny are responsible for the cyclical renewal of hair follicles and maintenance of the hair coat. The understanding of pathways involved in this process is essential to elucidate the pathogenetic mechanisms of primary alopecia. Stem cells and their direct descendants are located in the bulge region of the isthmus of hair follicles. Although these cells have been studied extensively in mice and humans, data for canine isthmic keratinocyte activation and proliferation are not available.
HYPOTHESIS/OBJECTIVES: The aim was to establish an accurate and reliable in vitro system to study the growth potential of canine isthmic keratinocytes. We assessed the colony-promoting capability of a commercially available canine-specific medium, CELLnTEC (CnT-09), compared with a well-established home-made medium, complete FAD (cFAD). The CnT-09 medium is specific for the growth of canine keratinocytes, while the cFAD medium can support growth and colony formation of keratinocytes from several species.
Skin biopsies were obtained from 15 recently euthanized dogs of various breeds with no skin abnormalities.
The isthmic region of compound hair follicles was isolated by microdissection and cell growth monitored using several parameters with colony-forming assays.
The CnT-09 and cFAD media provided similar growth as measured by the total number and size of colonies, as well as rate of cell differentiation.
The commercial canine-specific CnT-09 medium was comparable to the home-made cFAD medium in supporting the growth and proliferation of canine follicular keratinocytes in vitro. The CnT-09 medium should be a viable alternative growth medium for molecular studies of alopecic disorders in dogs.
毛囊干细胞及其子代负责毛囊的周期性更新和毛发的维持。了解这一过程中涉及的通路对于阐明原发性脱发的发病机制至关重要。干细胞及其直接后代位于毛囊峡部的隆突区。尽管这些细胞在小鼠和人类中已得到广泛研究，但关于犬峡部角质形成细胞激活和增殖的数据尚不可得。
假设/目的：目的是建立一个准确可靠的体外系统来研究犬峡部角质形成细胞的生长潜能。我们评估了一种市售的犬专用培养基CELLnTEC（CnT - 09）与一种成熟的自制培养基完全FAD（cFAD）相比促进集落形成的能力。CnT - 09培养基对犬角质形成细胞的生长具有特异性，而cFAD培养基可支持多种物种角质形成细胞的生长和集落形成。
从15只近期安乐死的无皮肤异常的不同品种犬获取皮肤活检样本。
通过显微切割分离复合毛囊的峡部区域，并使用集落形成试验中的几个参数监测细胞生长。
通过集落总数、大小以及细胞分化率来衡量，CnT - 09和cFAD培养基提供了相似的生长情况。
市售的犬专用CnT - 09培养基在体外支持犬毛囊角质形成细胞的生长和增殖方面与自制的cFAD培养基相当。CnT - 09培养基应是用于犬脱发疾病分子研究的一种可行的替代生长培养基。
