Buchweitz John, McClure-Brinton Kimberly, Zyskowski Justin, Stensen Lauren, Lehner Andreas
Diagnostic Center for Population and Animal Health, Michigan State University, 4125 Beaumont Rd, Lansing, MI 48910, United States; Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Rd, East Lansing, MI 48824, United States.
Country Companion Veterinary Services, 116 Old Amity Rd, Bethany, CT 06524, United States.
Regul Toxicol Pharmacol. 2015 Mar;71(2):174-7. doi: 10.1016/j.yrtph.2014.12.015. Epub 2014 Dec 26.
Lead (Pb) is a common cause of heavy metal poisonings in cattle. Sources of Pb on farms include crankcase oil, machinery grease, batteries, plumbing, and paint chips. Consequently, consumption of Pb from these sources may negatively impact animal health and Pb may be inadvertently introduced into the food supply. Therefore, the scope of poisoning incidents must be clearly assessed and sources of intoxication identified and strategies to mitigate exposure evaluated and implemented to prevent future exposures. Stable isotope analysis by inductively-coupled plasma mass spectrometry (ICP-MS) has proven itself of value in forensic investigations. We report on the extension of Pb stable isotope analysis to bovine tissues and profile comparisons with paint chips and soils collected from an affected dairy farm to elucidate the primary source. Pb occurs naturally as four stable isotopes: (204)Pb, (206)Pb, (207)Pb, and (208)Pb. Herein a case is reported to illustrate the use of (207)Pb/(206)Pb and (208)Pb/(206)Pb ratios to link environmental sources of exposure with tissues from a poisoned animal. Chemical Pb profiling provides a valuable tool for field investigative approaches to Pb poisoning in production agriculture and is applicable to subclinical exposures.
铅（Pb）是牛重金属中毒的常见原因。农场中铅的来源包括曲轴箱油、机械润滑脂、电池、管道和油漆碎片。因此，从这些来源摄入铅可能会对动物健康产生负面影响，并且铅可能会无意中进入食物供应中。因此，必须明确评估中毒事件的范围，确定中毒源，并评估和实施减轻接触的策略，以防止未来的接触。通过电感耦合等离子体质谱法（ICP-MS）进行的稳定同位素分析已在法医调查中证明了其价值。我们报告了将铅稳定同位素分析扩展到牛组织，并与从受影响的奶牛场收集的油漆碎片和土壤进行剖面比较，以阐明主要来源。铅天然以四种稳定同位素的形式存在：（204）Pb、（206）Pb、（207）Pb和（208）Pb。本文报告了一个案例，以说明使用（207）Pb/（206）Pb和（208）Pb/（206）Pb比率将环境接触源与中毒动物的组织联系起来。化学铅剖析为生产农业中铅中毒的现场调查方法提供了一个有价值的工具，并且适用于亚临床接触。
