Campbell A E, Slater J S, Futch W S
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
Virology. 1989 Nov;173(1):268-75. doi: 10.1016/0042-6822(89)90243-2.
Antigen-specific cytotoxic T lymphocyte (CTL) maturation was inhibited in mice acutely infected with murine cytomegalovirus (MCMV). When immunization with Simian virus 40 (SV40) either preceded or followed infection with MCMV by 1 day, the frequency of SV40-specific CTL precursors among lymph node cells (LNC) was significantly reduced compared to noninfected mice. Replication of the herpesvirus in LNC could not be detected; however, MCMV rendered noninfectious by heat treatment was not suppressive to CTL development. Lymph node cells from nonimmunized, MCMV-infected mice contained a cell(s) present in low frequency which suppressed in vitro maturation of SV40 CTL in immune lymph nodes from mice not infected with MCMV. These suppressor cells did not affect the antigen- and interleukin-2-dependent growth nor cytotoxic activity of a mature, SV40-specific CTL clone. These results indicate that MCMV interferes with immunoregulatory functions required for development of antigen-specific CTL precursors to mature effector CTL. The immunosuppression is mediated at least in part by the MCMV-induced suppressor cell(s).
在急性感染鼠巨细胞病毒（MCMV）的小鼠中，抗原特异性细胞毒性T淋巴细胞（CTL）的成熟受到抑制。当用猴病毒40（SV40）免疫在感染MCMV之前或之后1天进行时，与未感染的小鼠相比，淋巴结细胞（LNC）中SV40特异性CTL前体的频率显著降低。未检测到疱疹病毒在LNC中的复制；然而，经热处理使其失去感染性的MCMV对CTL的发育没有抑制作用。来自未免疫、MCMV感染小鼠的淋巴结细胞含有低频存在的一种或多种细胞，这些细胞在体外抑制了未感染MCMV小鼠免疫淋巴结中SV40 CTL的成熟。这些抑制细胞不影响成熟的、SV40特异性CTL克隆的抗原和白细胞介素2依赖性生长及细胞毒性活性。这些结果表明，MCMV干扰了抗原特异性CTL前体发育为成熟效应CTL所需的免疫调节功能。免疫抑制至少部分是由MCMV诱导的抑制细胞介导的。
