Bastow K F, Zhou B S, Cheng Y C
Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill 27599-7365.
Virus Genes. 1989 Aug;2(4):357-65. doi: 10.1007/BF00684043.
A cDNA for human topoisomerase I (Topo 1) was used to identify a 4.1 kb polyadenylated Topo 1 mRNA in methotrexate-resistant human KB cells that are permissive for herpes simplex virus type 2 (HSV-2) infection. Using these cells, no effect of the HSV-2-associated early shut-off function on levels of Topo-1 mRNA was observed up to 6 hours postinfection, whereas the actin mRNA level was 22% cellular transcripts are susceptible. The level of several host-cell polyadenylated RNAs detected as cDNA clones (class 3 transcripts) were unchanged 8 hours after HSV-2 infection, and other cellular transcripts (class 2) actually accumulated at postinfection.
用人拓扑异构酶I（Topo 1）的互补DNA（cDNA），在对单纯疱疹病毒2型（HSV - 2）感染敏感的耐甲氨蝶呤人KB细胞中鉴定出一个4.1 kb的聚腺苷酸化Topo 1信使核糖核酸（mRNA）。利用这些细胞，在感染后长达6小时内，未观察到HSV - 2相关的早期关闭功能对Topo - 1 mRNA水平有影响，而肌动蛋白mRNA水平在感染后6小时是细胞转录本的22%。HSV - 2感染8小时后，作为cDNA克隆检测到的几种宿主细胞聚腺苷酸化RNA（3类转录本）水平未变，而其他细胞转录本（2类）在感染后实际有所积累。
