Stauch Kelly L, Purnell Phillip R, Villeneuve Lance M, Fox Howard S
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
Proteomics. 2015 May;15(9):1574-86. doi: 10.1002/pmic.201400277. Epub 2015 Feb 10.
Mitochondria are the main cellular source of reactive oxygen species and are recognized as key players in several age-associated disorders and neurodegeneration. Their dysfunction has also been linked to cellular aging. Additionally, mechanisms leading to the preservation of mitochondrial function promote longevity. In this study we investigated the proteomic and functional alterations in brain mitochondria isolated from mature (5 months old), old (12 months old), and aged (24 months old) mice as determinants of normal "healthy" aging. Here the global changes concomitant with aging in the mitochondrial proteome of mouse brain analyzed by quantitative mass-spectrometry based super-SILAC identified differentially expressed proteins involved in several metabolic pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Despite these changes, the bioenergetic function of these mitochondria was preserved. Overall, this data indicates that proteomic changes during aging may compensate for functional defects aiding in preservation of mitochondrial function. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD001370 (http://proteomecentral.proteomexchange.org/dataset/PXD001370).
线粒体是活性氧的主要细胞来源，被认为是几种与年龄相关的疾病和神经退行性变的关键因素。它们的功能障碍也与细胞衰老有关。此外，导致线粒体功能维持的机制可促进长寿。在本研究中，我们调查了从成熟（5个月大）、老年（12个月大）和高龄（24个月大）小鼠分离的脑线粒体中的蛋白质组学和功能改变，作为正常“健康”衰老的决定因素。在这里，通过基于定量质谱的超级稳定同位素标记氨基酸细胞培养技术（super-SILAC）分析的小鼠脑线粒体蛋白质组中与衰老相关的全局变化，确定了参与包括糖酵解、三羧酸循环和氧化磷酸化在内的几种代谢途径的差异表达蛋白。尽管有这些变化，这些线粒体的生物能量功能仍得以保留。总体而言，这些数据表明衰老过程中的蛋白质组学变化可能补偿功能缺陷，有助于维持线粒体功能。质谱蛋白质组学数据已存入蛋白质组交换联盟，数据集标识符为PXD001370（http://proteomecentral.proteomexchange.org/dataset/PXD001370）。
