通过在白腐真菌黄孢原毛平革菌YK-624中重组表达木糖还原酶提高木糖醇产量。
Improving xylitol production through recombinant expression of xylose reductase in the white-rot fungus Phanerochaete sordida YK-624.
作者信息
Hirabayashi Sho, Wang Jianqiao, Kawagishi Hirokazu, Hirai Hirofumi
机构信息
Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
出版信息
J Biosci Bioeng. 2015 Jul;120(1):6-8. doi: 10.1016/j.jbiosc.2014.11.024. Epub 2014 Dec 26.
We generated an expression construct consisting of the xylose reductase (XR) gene (xr) from Phanerochaete chrysosporium. Transformant X7 exhibited increased xylitol production and markedly higher XR activities than the wild-type strain. RT-PCR analysis demonstrated that the increased XR activity was associated with constant expression of the recombinant xr gene.
我们构建了一个表达载体，其包含来自黄孢原毛平革菌的木糖还原酶（XR）基因（xr）。转化体X7与野生型菌株相比，木糖醇产量增加且XR活性显著更高。逆转录聚合酶链反应（RT-PCR）分析表明，XR活性的增加与重组xr基因的持续表达相关。
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