Wang Jianqiao, Hirabayashi Sho, Mori Toshio, Kawagishi Hirokazu, Hirai Hirofumi
Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
J Biosci Bioeng. 2016 Jul;122(1):17-21. doi: 10.1016/j.jbiosc.2015.12.002. Epub 2016 Jan 4.
To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.
为提高黄孢原毛平革菌YK-624的乙醇产量，从这种高效降解木质素的真菌中克隆了丙酮酸脱羧酶（PDC）基因，并将其重新导入该真菌；该基因编码酒精发酵中的一种关键酶。我们筛选了16株转化型黄孢原毛平革菌YK-624菌株，每株都表达第二个重组PDC基因（pdc），然后鉴定出乙醇产量最高的转化菌株（命名为GP7）。与野生型黄孢原毛平革菌YK-624相比，GP7从阔叶木中直接生产乙醇的能力提高了1.41倍。逆转录聚合酶链反应（RT-PCR）分析表明，重组pdc表达量的提高导致了PDC活性的增加。综上所述，这些结果表明，通过稳定表达额外的重组pdc，可以提高黄孢原毛平革菌YK-624的乙醇产量。
