Côrtes Marina Farrel, Beltrame Cristiana Ossaille, Ramundo Mariana Severo, Ferreira Fabienne Antunes, Figueiredo Agnes Marie Sá
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil.
Int J Med Microbiol. 2015 Jan;305(1):140-7. doi: 10.1016/j.ijmm.2014.11.011. Epub 2014 Dec 5.
Biofilm formation is considered an important virulence factor in implanted device-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Recent studies demonstrated that the ica-independent biofilms produced by MRSA are multifactorial. Despite the recent progress achieved in this field, the bacterial factors associated with biofilm formation/accumulation and regulation among clinical MRSA isolates remain largely unknown. In this study, using MRSA isolates from diverse multilocus sequence typing (MLST) clonal complexes that produce different amounts of biofilm, and a number of phenotypic and molecular approaches, we investigated the correlation between biofilm-associated factors and the ability of the bacteria to accumulate biofilm.
生物膜形成被认为是耐甲氧西林金黄色葡萄球菌（MRSA）引起的植入装置相关感染中的一个重要毒力因子。最近的研究表明，MRSA产生的ica非依赖性生物膜是多因素的。尽管该领域最近取得了进展，但临床MRSA分离株中与生物膜形成/积累及调控相关的细菌因素仍 largely未知。在本研究中，我们使用来自不同多位点序列分型（MLST）克隆复合体的MRSA分离株，这些分离株产生不同量的生物膜，并采用了多种表型和分子方法，研究了生物膜相关因子与细菌积累生物膜能力之间的相关性。
