Machado Thamiris Santana, Pinheiro Felipe Ramos, Andre Lialyz Soares Pereira, Pereira Renata Freire Alves, Correa Reginaldo Fernandes, de Mello Gabriela Coutinho, Ribeiro Tainara Aparecida Nunes, Penna Bruno, Sachs Daniela, Aguiar-Alves Fábio
Biotechnology and Molecular Epidemiology Laboratory, Fluminense Federal University, Niteroi, 24220-900 Rio de Jeneiro, Brazil.
Pathology Postgraduate Program, Fluminense Federal University, Niteroi, 24220-900 Rio de Janeiro, Brazil.
Toxins (Basel). 2020 Dec 25;13(1):14. doi: 10.3390/toxins13010014.
Hospitalizations related to Methicillin-resistant (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói-Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the staphylococcal chromosomal cassette (SCC), A, Panton-Valentine Leucocidin (PVL), C, R, ACME, and virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCC type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCC type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.
与耐甲氧西林金黄色葡萄球菌（MRSA）相关的住院情况频繁发生，增加了死亡率和医疗成本。因此，本研究旨在比较在巴西里约热内卢尼泰罗伊市两家医院就诊的定植和感染患者的MRSA分离株的基因型和表型特征。对2013年3月至2015年12月期间收集的147份样本进行表型和基因分型，以鉴定蛋白A（SPA）基因、葡萄球菌染色体盒式元件（SCC）、A、杀白细胞素（PVL）、C、R、ACME和毒力基因。还研究了生物膜形成的强度。在定植组中观察到IV型SCC的患病率为77.1%；然而，在侵袭性感染组中，II型SCC最为常见（62.9%）。多位点序列分型（MLST）分析中，ST5/ST30和ST5/ST239分别是定植和侵袭性感染分离株中最常见的克隆。在选择用于评估生物膜形成能力的分离株中，51.06%被归类为强生物膜形成者。令人惊讶的是，我们观察到巴西医院中出现了非巴西流行克隆（BEC）的分离株。由于在该样本集中也鉴定出了I型和II型ACME基因，这些分离株的毒力谱发生了变化。
