Nopper B, Kohen F, Wilchek M
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Anal Biochem. 1989 Jul;180(1):66-71. doi: 10.1016/0003-2697(89)90088-2.
Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of high capacity and is suitable for the rapid and single-step purification of all subclasses of monoclonal and polyclonal antibodies. Due to its broad specificity, the thiophilic silica column is an efficient, stable, and inexpensive substitute for protein A and protein G columns used today to purify antibodies.
亲硫吸附色谱法最初由波拉特及其同事（1985年，《欧洲生物化学学会联合会快报》185卷，306 - 310页）开发用于传统色谱技术，在制备新型改良含硫硅胶珠后转变为高效液相色谱模式。这种新型亲硫吸附剂具有高容量，适用于单克隆和多克隆抗体所有亚类的快速一步纯化。由于其广泛的特异性，亲硫硅胶柱是目前用于纯化抗体的蛋白A柱和蛋白G柱的一种高效、稳定且廉价的替代品。
