不育男性精子活力与腺苷酸环化酶活性之间的相关性。

Correlation between the sperm motility and the adenylate cyclase activity in infertile men.

作者信息

Ishikawa H, Tomomasa H, Yoshii S, Koiso K, Tajima Y, Okamura N, Sugita Y

机构信息

Department of Urology, University of Tsukuba, Ibaraki, Japan.

出版信息

Andrologia. 1989 Sep-Oct;21(5):437-40.

PMID:2554762

Abstract

The relationship between the sperm motility and the adenylate cyclase activity in spermatozoa from patients with male infertility was investigated. Cyclic AMP contents were measured from 34 semen specimens and adenylate cyclase activity were assayed from 50 specimens. All semen specimens were collected from infertile men at our clinic by masturbation after 5 days sexual abstinence. Spermatozoa were washed and concentrated according to the method described by Harrison. The cAMP contents and the adenylate cyclase activity of spermatozoa were determined by radioimmunoassay. Positive correlations were found among the cAMP contents, the adenylate cyclase activity in human spermatozoa and the sperm motility. This result suggests that the sperm motility is regulated by the adenylate cyclase activity via cAMP and that poor sperm motility observed in infertile men is partially caused by the impairment of adenylate cyclase system.

摘要

对男性不育患者精子的运动能力与腺苷酸环化酶活性之间的关系进行了研究。检测了34份精液标本中的环磷酸腺苷（cAMP）含量，并对50份标本进行了腺苷酸环化酶活性测定。所有精液标本均为在我诊所就诊的不育男性在禁欲5天后通过手淫采集。按照哈里森描述的方法对精子进行洗涤和浓缩。通过放射免疫测定法测定精子的cAMP含量和腺苷酸环化酶活性。发现人精子中的cAMP含量、腺苷酸环化酶活性与精子运动能力之间呈正相关。这一结果表明，精子运动能力受腺苷酸环化酶活性通过cAMP的调节，不育男性中观察到的精子运动能力差部分是由腺苷酸环化酶系统受损所致。