Adeleke Matthew Adekunle, Peters Sunday Olusola, Ogunmodede Dare Tunde, Oni Oluwole Oyetunde, Ajayi Olusola Lawrence, Wheto Mathew, Adebambo Olufunmilayo Ayoka
Department of Animal Breeding and Genetics, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State, Nigeria.
Trop Anim Health Prod. 2015 Feb;47(2):391-4. doi: 10.1007/s11250-014-0728-9. Epub 2014 Dec 30.
This study was conducted to evaluate the variation in antibody from Newcastle disease (ND) vaccinated breeder birds and their progenies. The chicks were evaluated for maternally derived antibody (MDA) against ND virus and also the MDA transfer rate for each genotype. The local parent stocks were Frizzle-feathered, Normal-feathered, and Naked neck while an exotic parent stock (Anak Titan) was used for the purpose of comparison. Sera samples were collected from chicks generated at day 3 post-hatch, and sera were also collected from the parent stocks. The sera samples were stored at -20 °C until analysed. Enzyme linked immuno-sorbent assay (ELISA) test was used for the detection and quantification of antibodies to ND in the parent stocks and the chicks using IDEXX Newcastle Disease ELISA test kit. The least squares means of antibody titers for parent stocks were 413.333 ± 68.853, 426.333 ± 121.949, 481.667 ± 145.454, and 1148.333 ± 532.843 for Frizzle-feathered, Normal-feathered, Naked Neck, and Anak Titan, and the calculated MDA transfer rate was 96.4, 93.8, 88.7, and 34.7 % for Frizzle-feathered, Normal-feathered, Naked neck, and Anak Titan, respectively. The mean antibody titers for chicks were 398.6 ± 43.871, 400.0 ± 53.952, 427.0 ± 89.353, and 398.8 ± 57.593 for Frizzle-feathered, Normal-feathered, Naked neck, and Anak Titan, respectively. The antibody titers from parent stocks were higher than those observed in chicks for all the genotypes. The values obtained in this study indicated that parent stocks with their progenies were below protective level. The local parent stocks had higher transfer rate when compared to their exotic counterpart. Hence, there is an indication from this finding that the local chickens had a better transfer rate in relation to Anak Titan which is an exotic breed. Therefore, there is the presence of genetic variability for antibody transfer in the Nigerian local chickens and can be incorporated in designing a breeding program for better adaptive potential.
本研究旨在评估接种新城疫（ND）疫苗的种鸡及其后代抗体的变化情况。对雏鸡进行了针对新城疫病毒的母源抗体（MDA）评估以及每种基因型的MDA转移率评估。本地亲本种群包括卷羽鸡、正常羽鸡和裸颈鸡，同时使用外来亲本种群（阿纳克泰坦鸡）作为对照。在雏鸡出壳后第3天采集血清样本，也从亲本种群采集血清样本。血清样本保存在-20°C直至分析。使用IDEXX新城疫ELISA检测试剂盒，通过酶联免疫吸附测定（ELISA）试验检测和定量亲本种群及雏鸡中新城疫抗体。卷羽鸡、正常羽鸡、裸颈鸡和阿纳克泰坦鸡亲本种群抗体滴度的最小二乘均值分别为413.333±68.853、426.333±121.949、481.667±145.454和1148.333±532.843，计算得出的卷羽鸡、正常羽鸡、裸颈鸡和阿纳克泰坦鸡的MDA转移率分别为96.4%、93.8%、88.7%和34.7%。卷羽鸡、正常羽鸡、裸颈鸡和阿纳克泰坦鸡雏鸡的平均抗体滴度分别为398.6±43.871、400.0±53.952、427.0±89.353和398.8±57.593。所有基因型亲本种群的抗体滴度均高于雏鸡中的观察值。本研究获得的值表明亲本种群及其后代低于保护水平。与外来亲本种群相比，本地亲本种群的转移率更高。因此，这一发现表明，与外来品种阿纳克泰坦鸡相比，本地鸡的转移率更好。所以，尼日利亚本地鸡在抗体转移方面存在遗传变异性，可纳入育种计划设计中以获得更好的适应潜力。
