Liu Shu-Guang, Yuan Shuang-Hu, Wu Hui-Yong, Liu Jie, Huang Cheng-Suo
Departments of Surgery, Shandong Cancer Hospital and Institute, Jinan, 250117, Shandong, People's Republic of China.
Departments of Radiotherapy, Shandong Cancer Hospital and Institute, Jinan, 250117, Shandong, People's Republic of China.
Cell Biochem Biophys. 2015 May;72(1):165-9. doi: 10.1007/s12013-014-0431-5.
The aim of the study was to analyze the expressions of vascular endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1) and endostatin in non-small cell lung caner (NSCLC), and to explore their correlations with NSCLC. 80 NSCLC patients during January 2013 to June 2014 were selected. The expression levels of VEGF, TGF-β1, and endostatin before surgeries were detected, and compared with 40 healthy individuals and 40 patients with benign pulmonary diseases. Serum VEGF, TGF-β1, and endostatin levels were (573.6 ± 25.4) pg/mL, (36.2 ± 10.5) ng/mL, and (20.3 ± 7.8) ng/mL, respectively, in NSCLC group, which were obviously higher than those in healthy individuals and patients with benign pulmonary diseases, and the difference was statistically significant (p < 0.05); there was no statistical difference of the VEGF, TGF-β1, and endostatin levels between the healthy individuals and patients with benign pulmonary diseases (p > 0.05), or among patients with different physiological characteristics such as gender, age, and smoking history in NSCLC group (p > 0.05). Serum VEGF, TGF-β1, and endostatin levels also showed no statistical difference among patients with different pathological characteristics such as histological types, with or without lymphatic metastasis (p > 0.05). However, the three indicators were significantly different among patients with different TNM stages, with or without distant metastasis and different cell differentiation degrees (p < 0.05). Serum VEGF and TGF-β1 were positively related (r = 0.479, p < 0.05), endostatin was negatively related to both VEGF and TGF-β1 (r = -0.392, -0.354, p < 0.05 in both comparisons). The expression levels of VEGF, TGF-β1, and endostatin significantly contributed to the poor cell differentiation in NSCLC. They had important effects on the occurrence, development, and metastasis of NSCLC, which could be applied as the indicators to predict the malignancy of NSCLC.
本研究旨在分析血管内皮生长因子（VEGF）、转化生长因子β1（TGF-β1）和内皮抑素在非小细胞肺癌（NSCLC）中的表达情况，并探讨它们与NSCLC的相关性。选取2013年1月至2014年6月期间的80例NSCLC患者。检测手术前VEGF、TGF-β1和内皮抑素的表达水平，并与40例健康个体和40例良性肺部疾病患者进行比较。NSCLC组血清VEGF、TGF-β1和内皮抑素水平分别为（573.6±25.4）pg/mL、（36.2±10.5）ng/mL和（20.3±7.8）ng/mL，明显高于健康个体和良性肺部疾病患者，差异有统计学意义（p<0.05）；健康个体与良性肺部疾病患者之间VEGF、TGF-β1和内皮抑素水平无统计学差异（p>0.05），NSCLC组不同生理特征（如性别、年龄和吸烟史）的患者之间也无统计学差异（p>0.05）。不同病理特征（如组织学类型、有无淋巴转移）的患者之间血清VEGF、TGF-β1和内皮抑素水平也无统计学差异（p>0.05）。然而，这三项指标在不同TNM分期、有无远处转移和不同细胞分化程度的患者之间有显著差异（p<0.05）。血清VEGF与TGF-β1呈正相关（r=0.479，p<0.05），内皮抑素与VEGF和TGF-β1均呈负相关（两次比较r分别为-0.392、-0.354，p均<0.05）。VEGF、TGF-β1和内皮抑素的表达水平显著导致NSCLC细胞分化不良。它们对NSCLC的发生、发展和转移有重要影响，可作为预测NSCLC恶性程度的指标。
