Price Philip J R, Bánki Zoltán, Scheideler Angelika, Stoiber Heribert, Verschoor Admar, Sutter Gerd, Lehmann Michael H
Institut für Infektionsmedizin und Zoonosen, Ludwig-Maximilians-Universität München, Munich 80539, Germany; German Centre for Infection Research, Partner Site Munich, Munich 80539, Germany;
Division of Virology, Medical University of Innsbruck, Innsbruck 6020, Austria;
J Immunol. 2015 Feb 1;194(3):1164-8. doi: 10.4049/jimmunol.1301410. Epub 2014 Dec 29.
Efficient leukocyte migration is important for an effective host response to viral infection and the development of adaptive immunity. The poxvirus strain modified vaccinia virus Ankara (MVA), a safe and efficient viral vector, rapidly induces chemokine expression and respiratory recruitment of leukocytes, which is unique among vaccinia viruses. In addition to chemokines, the complement system contributes to the attraction and activation of different types of leukocytes. Using a murine model of intranasal infection, we show in this study that MVA-induced neutrophil recruitment depends on complement component C5. Remarkably, we find that C5 mediates neutrophil recruitment to the lung, even in the absence of the central complement component C3. Our findings argue for complement C5 activation during MVA infection of the lung via a C3-independent pathway, which enables rapid recruitment of neutrophils.
有效的白细胞迁移对于宿主对病毒感染的有效反应以及适应性免疫的发展至关重要。痘病毒株安卡拉改良痘苗病毒（MVA）是一种安全有效的病毒载体，能迅速诱导趋化因子表达和白细胞的呼吸道募集，这在痘苗病毒中是独一无二的。除趋化因子外，补体系统也有助于不同类型白细胞的吸引和激活。在本研究中，我们利用鼻内感染的小鼠模型表明，MVA诱导的中性粒细胞募集依赖于补体成分C5。值得注意的是，我们发现即使在没有核心补体成分C3的情况下，C5也能介导中性粒细胞向肺部募集。我们的研究结果表明，在肺部MVA感染期间，补体C5通过不依赖C3的途径被激活，从而能够快速募集中性粒细胞。
