一种简化HIV gp 120 CD4结合区域定点诱变的策略。
A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120.
作者信息
Hemming A, Lundberg L, Olofsson S
机构信息
Department of Clinical Virology, University of Göteborg, Sweden.
出版信息
Arch Virol. 1989;107(3-4):301-5. doi: 10.1007/BF01317925.
PMID:2554859
Abstract
By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for further mutagenesis and is also easy to reclone.
摘要
通过定点诱变，在HIV gp 120的CD4结合DNA区域周围创建了两个独特的限制性酶切位点。这些突变不会导致氨基酸替换，但会产生一个DNA片段，该片段克隆到M13中进行进一步诱变时很稳定，并且也易于再次克隆。
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