Prelle A, Moggio M, Fagiolari G, Scarlato G, Miranda A, Bonilla E
Istituto di Clinica Neurologica Centro Dino Ferrari, Università di Milano, Italy.
Basic Appl Histochem. 1989;33(3):179-83.
We have performed immunofluorescent and fluorescent in situ hybridization studies in order to better clarify the integration of SV40 DNA in human fibroblast cell lines. Most of the cells were T-antigen positive by immunocytochemical studies, while in all the cells we detected the integrated viral DNA by in situ hybridization. Both techniques are easy and useful to perform but the molecular genetic method gives a more specific signal with the possibility of localizing molecular hybrids in the nucleus and in the cytoplasm of the transformed cells.
为了更好地阐明SV40 DNA在人成纤维细胞系中的整合情况，我们进行了免疫荧光和荧光原位杂交研究。通过免疫细胞化学研究，大多数细胞呈T抗原阳性，而通过原位杂交，我们在所有细胞中都检测到了整合的病毒DNA。这两种技术操作简便且有用，但分子遗传学方法能给出更特异的信号，并且有可能在转化细胞的细胞核和细胞质中定位分子杂交体。
