Marlhens F, Saint-Ruf C, Nardeux P, Lavialle C, Brouty-Boye D, Dutrillaux B, Cassingena R
C.N.R.S.-U.A. 620, Institut Curie, Paris, France.
Ann Genet. 1988;31(2):81-6.
Cytogenetic analysis of the human HBL-100 cell line, that we have previously shown to harbour SV40 genetic information (Caron de Fromentel et al., 1985), reveals numerous chromosomal rearrangements as soon as the 30th in vitro passage. The karyotype is relatively stable during in vitro maintenance and even at late passages (approximately 70) when the cells have acquired the capacity to form tumors in nude mice. In all the somatic cell hybrids obtained after fusion of mouse 3T3-4E cells with HBL-100 cells, several human chromosomes are maintained and a derivative from chromosome 15-der(15)- is the most frequently observed. The der(15) marker is present in the HBL-100 cell line at every passage studied as well as in different cell lines derived from tumors induced by HBL-100 cells. The various hybrids, originally isolated for a transformed phenotype on the basis of their ability to grow in soft-agar, were all found to express the SV40 T-antigen. In situ hybridization of an SV40 DNA probe to chromosome spreads obtained from one of these hybrids shows that the integration site of the viral genome is located on the der(15) marker chromosome, at band 15q24. The possible cooperation of SV40 T-antigen with some other oncogene(s), required by human HBL-100 cells in order to express a malignant phenotype, is discussed.
我们之前已证明人类HBL - 100细胞系含有SV40遗传信息（Caron de Fromentel等人，1985年），对其进行的细胞遗传学分析显示，在体外传代第30次时就出现了大量染色体重排。在体外培养过程中，甚至在后期传代（约70代）时，当细胞获得在裸鼠体内形成肿瘤的能力时，核型相对稳定。在小鼠3T3 - 4E细胞与HBL - 100细胞融合后获得的所有体细胞杂种中，保留了几条人类染色体，其中15号染色体的衍生物der(15)最为常见。在所研究的HBL - 100细胞系的每一代以及由HBL - 100细胞诱导产生的肿瘤衍生的不同细胞系中都存在der(15)标记。最初根据其在软琼脂中生长的能力分离出的各种杂种，均被发现表达SV40 T抗原。用SV40 DNA探针与从其中一个杂种获得的染色体铺片进行原位杂交，结果表明病毒基因组的整合位点位于der(15)标记染色体的15q24带。本文讨论了人类HBL - 100细胞为表达恶性表型所需的SV40 T抗原与其他一些癌基因可能的协同作用。
