Oyarbide Usua, Iturria Iñaki, Rainieri Sandra, Pardo Miguel Angel
Food Research Division, Azti-Tecnalia , Derio, Spain .
Zebrafish. 2015 Feb;12(1):71-80. doi: 10.1089/zeb.2014.0972. Epub 2014 Dec 30.
We evaluated the use of the gnotobiotic zebrafish system to study the effects of bacterial infection, and analyzed expression of genes involved in zebrafish innate immunity. Using a GFP-labeled strain of Vibrio anguillarum, we fluorescently monitored colonization of the zebrafish intestinal tract and used gene expression analysis to compare changes in genes involved in innate immunity between nongnotobiotic and gnotobiotic larvae. The experiments performed with the gnotobiotic zebrafish reveal new insights into V. anguillarum pathogenesis. Specifically, an alteration of the host immune system was detected through the suppression of a number of innate immune genes (NFKB, IL1B, TLR4, MPX, and TRF) during the first 3 h post infection. This immunomodulation can be indicative of a "stealth mechanism" of mucus invasion in which the pathogen found a sheltered niche, a typical trait of intracellular pathogens.
我们评估了无菌斑马鱼系统在研究细菌感染影响方面的应用，并分析了斑马鱼先天免疫相关基因的表达。使用绿色荧光蛋白（GFP）标记的鳗弧菌菌株，我们通过荧光监测了斑马鱼肠道的定殖情况，并利用基因表达分析比较了无菌和非无菌幼虫中先天免疫相关基因的变化。使用无菌斑马鱼进行的实验揭示了鳗弧菌致病机制的新见解。具体而言，在感染后的前3小时内，通过抑制许多先天免疫基因（NFKB、IL1B、TLR4、MPX和TRF）检测到宿主免疫系统的改变。这种免疫调节可能表明存在一种黏液侵袭的“隐形机制”，即病原体找到了一个受保护的微环境，这是细胞内病原体的典型特征。
