松果体毒蕈碱磷酸肌醇反应：具有极低受体数量的百日咳毒素抗性信号传导。
Pineal muscarinic phosphoinositide response: pertussis toxin resistant signaling with very low receptor number.
作者信息
Laitinen J T, Torda T, Saavedra J M
机构信息
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892.
出版信息
Biochem Biophys Res Commun. 1989 Oct 31;164(2):645-52. doi: 10.1016/0006-291x(89)91509-x.
Autoradiography revealed very low density of muscarinic receptors in the rat pineal gland. Yet, the magnitude of phosphoinositide hydrolysis elicited with 0.1 mM carbachol was similar to that seen with 1 mM norepinephrine. The cholinergic and adrenergic phosphoinositide responses were fully additive. The cholinergic signal was insensitive to pertussis toxin both in vivo and in vitro and persisted in pineals cultured for 5 days. The data expand our previous finding on functional muscarinic acetylcholine receptors in the rat pineal gland.
放射自显影显示，大鼠松果体中的毒蕈碱受体密度极低。然而，0.1 mM卡巴胆碱引起的磷酸肌醇水解程度与1 mM去甲肾上腺素引起的相似。胆碱能和肾上腺素能磷酸肌醇反应完全相加。胆碱能信号在体内和体外对百日咳毒素均不敏感，并且在培养5天的松果体中仍然存在。这些数据扩展了我们之前关于大鼠松果体中功能性毒蕈碱型乙酰胆碱受体的发现。
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