Griffith Elizabeth C, Perkins Russell J, Telesford Dana-Marie, Adams Ellen M, Cwiklik Lukasz, Allen Heather C, Roeselová Martina, Vaida Veronica
†Department of Chemistry and Biochemistry and CIRES, University of Colorado at Boulder, UCB 215, Boulder, Colorado 80309, United States.
‡Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, United States.
J Phys Chem B. 2015 Jul 23;119(29):9038-48. doi: 10.1021/jp508473w. Epub 2014 Dec 30.
The interaction of L-phenylalanine with a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayer at the air-water interface was explored using a combination of experimental techniques and molecular dynamics (MD) simulations. By means of Langmuir trough methods and Brewster angle microscopy, L-phenylalanine was shown to significantly alter the interfacial tension and the surface domain morphology of the DPPC film. In addition, confocal microscopy was used to explore the aggregation state of L-phenylalanine in the bulk aqueous phase. Finally, MD simulations were performed to gain molecular-level information on the interactions of L-phenylalanine and DPPC at the interface. Taken together, these results show that L-phenylalanine intercalates into a DPPC film at the air-water interface, thereby affecting the surface tension, phase morphology, and ordering of the DPPC film. The results are discussed in the context of biological systems and the mechanism of diseases such as phenylketonuria.
运用实验技术与分子动力学（MD）模拟相结合的方法，探究了L-苯丙氨酸与空气-水界面处的1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）单分子层之间的相互作用。通过朗缪尔槽法和布鲁斯特角显微镜观察发现，L-苯丙氨酸能显著改变DPPC膜的界面张力和表面域形态。此外，利用共聚焦显微镜研究了L-苯丙氨酸在本体水相中的聚集状态。最后，进行了分子动力学模拟，以获取关于L-苯丙氨酸与DPPC在界面处相互作用的分子水平信息。综合这些结果表明，L-苯丙氨酸插入空气-水界面处的DPPC膜中，从而影响DPPC膜的表面张力、相形态和有序性。本文在生物系统以及苯丙酮尿症等疾病机制的背景下对这些结果进行了讨论。
