Sakurai K, Miura T
Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Biochem Int. 1989 Aug;19(2):405-12.
The interaction of alloxan with bovine serum albumin was studied. When alloxan was incubated with bovine serum albumin, oxygen consumption, H2O2 formation, and diminution of sulfhydryl groups of the protein were observed. During the reaction of alloxan with the protein, superoxide radicals were generated; and under anaerobic conditions, ESR signal of alloxan free radicals was observed. These results strongly suggest that alloxan mediates electron transfer from the protein sulfhydryl groups to oxygen.
研究了四氧嘧啶与牛血清白蛋白的相互作用。当四氧嘧啶与牛血清白蛋白一起温育时，观察到氧气消耗、过氧化氢生成以及蛋白质巯基减少。在四氧嘧啶与蛋白质的反应过程中，产生了超氧自由基；并且在厌氧条件下，观察到了四氧嘧啶自由基的电子自旋共振信号。这些结果有力地表明，四氧嘧啶介导了从蛋白质巯基到氧的电子转移。
