Anderson G J, Faulk W P, Arosio P, Moss D, Powell L W, Halliday J W
Department of Medicine, University of Queensland, Brisbane, Australia.
Br J Haematol. 1989 Oct;73(2):260-4. doi: 10.1111/j.1365-2141.1989.tb00262.x.
Monoclonal antibodies to the H and L subunits of ferritin were used in indirect immunofluorescent studies to investigate the subunit specificity of ferritin binding to transformed T and B lymphoid cell lines. Pre-incubation of cells with recombinant H ferritin followed by antibodies to the H subunit produced a labelling pattern in which all cells showed strong fluorescent stippling. Corresponding experiments with liver ferritin and an antibody to the L subunit revealed that most cells were lightly stippled, but 3-5% of the cells were intensely labelled. These two labelling patterns were distinct as assessed by cross-blocking experiments with recombinant H and liver ferritins. These results imply that different binding sites exist for each subunit.
使用针对铁蛋白H和L亚基的单克隆抗体进行间接免疫荧光研究，以探讨铁蛋白与转化的T和B淋巴细胞系结合的亚基特异性。用重组H铁蛋白预孵育细胞，然后用抗H亚基抗体处理，产生的标记模式是所有细胞均显示强烈的荧光斑点。用肝铁蛋白和抗L亚基抗体进行的相应实验表明，大多数细胞有轻微的斑点，但3-5%的细胞被强烈标记。通过用重组H铁蛋白和肝铁蛋白进行交叉阻断实验评估，这两种标记模式是不同的。这些结果表明每个亚基存在不同的结合位点。
