Li Ruixiong, Lan Bin, Zhu Tianxiang, Yang Yanlong, Wang Muting, Ma Chensheng, Chen Shu
Department of Cardiac-Thoracic Surgery, Shantou Central Hospital / Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China (mainland).
Med Sci Monit. 2014 Dec 31;20:2846-54. doi: 10.12659/MSM.891303.
Vascular restenosis occurring after CABG is a major clinical problem that needs to be addressed. Vein grafts are associated with a higher degree of stenosis than artery grafts. However, the mechanism responsible for this effect has not been elucidated. We aimed to establish a rabbit model of vascular restenosis after bilateral carotid artery grafting, and to investigate the associated spatiotemporal changes of intimal hyperplasia in carotid artery and jugular vein grafts after surgery.
Twenty adult New Zealand white rabbits (10 males; 10 females), weighing 2.0-2.5 kg, were obtained from the Experimental Animal Center of Southern Medical University, Guangzhou, China (License No.: scxk-Guangdong-2006-0015). We quantitatively analyzed intimal thickness, area, and degree of stenosis in carotid artery and jugular vein bridges.
After 8 weeks of a high-fat diet, rabbit carotid arteries showed early atherosclerotic lesions. With increasing time after surgery, carotid artery and jugular vein grafts showed histopathological and morphological changes, including smooth muscle cell migration, lipid deposition, intimal hyperplasia, and vascular stenosis. The degree of vascular stenosis was significantly higher in vein grafts than in artery grafts at all time points - 35.1±6.7% vs. 16.1±2.6% at Week 12, 56.2±8.5% vs. 23.4±3.4% at Week 16, and 71.2±1.3% vs. 25.2±5.3% at Week 20.
Rabbit bilateral carotid arteries were grafted with carotid artery and jugular vein bridges to simulate pathophysiological processes that occur in people after CABG surgery.
冠状动脉旁路移植术（CABG）后发生的血管再狭窄是一个需要解决的主要临床问题。静脉移植物比动脉移植物的狭窄程度更高。然而，造成这种影响的机制尚未阐明。我们旨在建立双侧颈动脉移植后血管再狭窄的兔模型，并研究术后颈动脉和颈静脉移植物内膜增生的相关时空变化。
从中国广州南方医科大学实验动物中心获得20只成年新西兰白兔（10只雄性；10只雌性），体重2.0 - 2.5千克（许可证号：scxk - 广东 - 2006 - 0015）。我们定量分析了颈动脉和颈静脉桥的内膜厚度、面积和狭窄程度。
高脂饮食8周后，兔颈动脉出现早期动脉粥样硬化病变。随着术后时间的增加，颈动脉和颈静脉移植物出现组织病理学和形态学变化，包括平滑肌细胞迁移、脂质沉积、内膜增生和血管狭窄。在所有时间点，静脉移植物的血管狭窄程度均显著高于动脉移植物——第12周时为35.1±6.7%对16.1±2.6%，第16周时为56.2±8.5%对23.4±3.4%，第20周时为71.2±I.3%对25.2±I.3%。
用颈动脉和颈静脉桥对兔双侧颈动脉进行移植，以模拟人类CABG手术后发生的病理生理过程。
