Xue Tao, Wei Li, Zhao Yu, Zha Ding-Jun, Qiao Li, Qiu Jian-Hua, Lu Lian-Jun
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University 17 Changle West Street, Xi'an 710032, Shanxi, P.R. China.
Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University 17 Changle West Street, Xi'an 710032, Shanxi, P.R. China.
Int J Clin Exp Pathol. 2014 Oct 15;7(11):7633-42. eCollection 2014.
NSCs/NPCs could be used for Sensorineural hearing loss treatment, because of the extensive capacity for self-renewal and pluripotency. In order to isolate and identify neural precursor cells (NPCs), we established a strategy to isolate and cultivate NPCs. Immunohistochemistry, immunofluorescence, Western blotting, and electron microscopy were used to characterize the cells and compare their differentiation patterns with those of olfactory bulb and olfactory epithelium NPCs. Furthermore, NPCs from the cochlear nucleus were sustained good cell viability and cloning efficiency after cryopreservation and thawing. Finally, high capacity to differentiate into astrocytes, oligodendrocytes, and neurons of NPCs was found. In conclusion, NPCs isolated from the cochlear nucleus can proliferate and differentiate into functional neurons, which offers a potential strategy for sensorineural hearing loss treatment. In addition, the storage method developed here will benefit further exploration of NPCs.
由于具有广泛的自我更新能力和多能性，神经干细胞/神经前体细胞可用于感音神经性听力损失的治疗。为了分离和鉴定神经前体细胞（NPCs），我们建立了一种分离和培养NPCs的策略。采用免疫组织化学、免疫荧光、蛋白质印迹和电子显微镜对细胞进行表征，并将其分化模式与嗅球和嗅上皮NPCs的分化模式进行比较。此外，来自耳蜗核的NPCs在冷冻保存和解冻后仍保持良好的细胞活力和克隆效率。最后，发现NPCs具有向星形胶质细胞、少突胶质细胞和神经元分化的高能力。总之，从耳蜗核分离的NPCs能够增殖并分化为功能性神经元，这为感音神经性听力损失的治疗提供了一种潜在策略。此外，这里开发的储存方法将有利于对NPCs的进一步探索。
