Stühmer W, Ruppersberg J P, Schröter K H, Sakmann B, Stocker M, Giese K P, Perschke A, Baumann A, Pongs O
Max-Planck-Institut für biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, FRG.
EMBO J. 1989 Nov;8(11):3235-44. doi: 10.1002/j.1460-2075.1989.tb08483.x.
Cloning and sequencing of cDNAs isolated from a rat cortex cDNA library reveals that a gene family encodes several highly homologous K+ channel forming (RCK) proteins. Functional characterization of the channels expressed in Xenopus laevis oocytes following microinjection of in vitro transcribed RCK-specific RNAs shows that each of the RCK proteins forms K+ channels that differ greatly in both their functional and pharmacological properties. This suggests that the molecular basis for the diversity of voltage-gated K+ channels in mammalian brain is based, at least partly, on the expression of several RCK proteins by a family of genes and their assembly to homooligomeric K+ channels with different functional properties.
从大鼠皮质cDNA文库中分离出的cDNA进行克隆和测序后发现，一个基因家族编码几种高度同源的钾离子通道形成（RCK）蛋白。在显微注射体外转录的RCK特异性RNA后，对非洲爪蟾卵母细胞中表达的通道进行功能表征，结果表明，每种RCK蛋白都形成钾离子通道，其功能和药理特性差异很大。这表明，哺乳动物大脑中电压门控钾离子通道多样性的分子基础至少部分基于一个基因家族对几种RCK蛋白的表达以及它们组装成具有不同功能特性的同型寡聚钾离子通道。
