Rudy B, Sen K, Vega-Saenz de Miera E, Lau D, Ried T, Ward D C
Department of Physiology and Biophysics, New York University Medical Center, NY 10016.
J Neurosci Res. 1991 Jul;29(3):401-12. doi: 10.1002/jnr.490290316.
Over ten different mammalian genes related to the Drosophila Shaker gene (the Sh gene family) have been identified recently. These genes encode subunits of voltage-dependent K+ channels. The family consists of four subfamilies: ShI genes are homologues of Shaker; ShII, ShIII, and ShIV are homologues of three other Shaker-like genes in Drosophila, Shab, Shaw, and Shal, respectively. We report here the cloning of a human K+ channel ShIII cDNA (HKShIIIC) obtained from a brain stem cDNA library. HKShIIIC transcripts express an atypical voltage-dependent transient (A-type) K+ current in Xenopus oocytes. This current is activated by large membrane depolarizations and is extremely sensitive to the K+ channel blocker TEA unlike most A-type currents. The gene encoding HKShIIIC maps to chromosome 1p21.
最近已经鉴定出超过十种与果蝇“震颤者”基因（Sh基因家族）相关的不同哺乳动物基因。这些基因编码电压依赖性钾离子通道的亚基。该家族由四个亚家族组成：ShI基因是“震颤者”基因的同源物；ShII、ShIII和ShIV分别是果蝇中另外三个类似“震颤者”基因Shab、Shaw和Shal的同源物。我们在此报告从脑干cDNA文库中获得的人类钾离子通道ShIII cDNA（HKShIIIC）的克隆。HKShIIIC转录本在非洲爪蟾卵母细胞中表达一种非典型的电压依赖性瞬态（A 型）钾电流。与大多数 A 型电流不同，这种电流由大幅度的膜去极化激活，并且对钾离子通道阻滞剂TEA极其敏感。编码HKShIIIC的基因定位于染色体1p21。
