Cajone F, Bernelli-Zazzera A
Istituto di Patologia Generale dell Universita degli Studi di Milano, Italy.
Free Radic Res Commun. 1989;7(3-6):189-94. doi: 10.3109/10715768909087941.
Hep G2 cells do not synthesize heat-shock proteins when incubated with ADP-iron, under conditions that can trigger lipoperoxidation. However, exposure of these cells to added 4-hydroxynonenal (HNE), one of the main products of lipoperoxidation, induces the synthesis of hsp70, the most conserved among heat-shock proteins. HNE acts mainly on transcription: in Hep G2 cells the increase in the steady-state level of hsp70 mRNA is detectable by two different hybridization techniques.
在能引发脂质过氧化的条件下，当与ADP-铁一起孵育时，Hep G2细胞不会合成热休克蛋白。然而，将这些细胞暴露于脂质过氧化的主要产物之一——添加的4-羟基壬烯醛（HNE）中，会诱导hsp70的合成，hsp70是热休克蛋白中最保守的一种。HNE主要作用于转录：在Hep G2细胞中，通过两种不同的杂交技术可检测到hsp70 mRNA稳态水平的增加。
