Ali Nasir, Ahmad Dawood, Bakht Jehan
Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Pak J Pharm Sci. 2015 Jan;28(1):195-200.
The present research work was carried out to investigate the antimicrobial (eight bacteria and one fungus) activities of different solvent (ethanol, petroleum ether, chloroform, ethyl acetate and isobutanol) extracted samples from flowers of P. obstusa by disc diffusion method. Analysis of the data revealed that all the five extracts from flowers of P. obstusa showed different ranges of antimicrobial activities. Petroleum ether fractions showed inhibitory activities against all the nine microbial species except Klebsiella pneumonia. Ethyl acetate and isobutanol fractions showed inhibitory effects against all the tested microbial species except Pseudomonas aeruginosa. Chloroform and ethanol extracts had varying levels of inhibitions against all of the tested microorganisms. The most susceptible gram positive bacterium was Bacillus subtilis which was inhibited by all the five extracts while the most resistant gram positive bacterium was Staphylococcus aureus. Erwinia carotovora was the most susceptible gram negative bacterium while Pseudomonas aeruginosa was highly resistant among the gram negative bacteria.
本研究旨在通过纸片扩散法研究从钝叶桂花朵中提取的不同溶剂(乙醇、石油醚、氯仿、乙酸乙酯和异丁醇)样品的抗菌(针对八种细菌和一种真菌)活性。数据分析表明,钝叶桂花朵的所有五种提取物均表现出不同程度的抗菌活性。石油醚馏分对除肺炎克雷伯菌外的所有九种微生物均有抑制活性。乙酸乙酯和异丁醇馏分对除铜绿假单胞菌外的所有测试微生物均有抑制作用。氯仿和乙醇提取物对所有测试微生物有不同程度的抑制作用。最敏感的革兰氏阳性菌是枯草芽孢杆菌,它受到所有五种提取物的抑制,而最耐药的革兰氏阳性菌是金黄色葡萄球菌。胡萝卜软腐欧文氏菌是最敏感的革兰氏阴性菌,而铜绿假单胞菌在革兰氏阴性菌中具有高度耐药性。