Bakht Jehan, Iftikhar Zainab, Shafi Mohammad, Iqbal Arshad
Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar KPK Pakistan.
Department of Agronomy, The University of Agriculture Peshawar KPK Pakistan.
Pak J Pharm Sci. 2017 Sep;30(5):1783-1789.
The present research was focused on the anti-microbial activities of different solvent extracted fractions from commercial available and fresh plants of Berberis lyceum against Gram positive, Gram-negative bacteria and fungi using 1 and 2 mg disc concentrations. Our results showed that fractions from both sources revealed different degree of antimicrobial activities. Our result indicated that Escherichia coli, Citorbacter freundii and Candida albicans were more susceptible to crude methanolic extract and the same microbes were resistant to water extracted fractions. Similarly, maximum reduction in the growth of Pseudomonas aeruginosa, Bacillus subtilis and Xanthomonas campestris was measured by hexane-extracted fractions and minimum growth inhibition by water-extracted fractions. Klebsiella pneumoniae and Staphylococcus aureus were more susceptible to ethyl acetate fraction. Majority of the tested microbes were resistant to water and butanol extracted samples. Staphylococcus aureus was the most susceptible gram-positive bacteria and Bacillus subtilis was resistant one. Among Gram-negative bacteria, Citorbacter freundii showed maximum susceptibility while Xanthomonas campestris revealed resistivity.
本研究聚焦于从商业可得的新鲜小檗植物中，使用1毫克和2毫克的纸片浓度，对不同溶剂萃取的部分进行抗革兰氏阳性菌、革兰氏阴性菌和真菌的活性研究。我们的结果表明，来自两种来源的部分均显示出不同程度的抗菌活性。我们的结果表明，大肠杆菌、弗氏柠檬酸杆菌和白色念珠菌对粗甲醇提取物更敏感，而相同的微生物对水提取物部分具有抗性。同样，通过己烷萃取部分测得铜绿假单胞菌、枯草芽孢杆菌和野油菜黄单胞菌的生长减少量最大，而水提取物部分的生长抑制最小。肺炎克雷伯菌和金黄色葡萄球菌对乙酸乙酯部分更敏感。大多数测试微生物对水和丁醇萃取样品具有抗性。金黄色葡萄球菌是最敏感的革兰氏阳性菌，枯草芽孢杆菌是抗性菌。在革兰氏阴性菌中，弗氏柠檬酸杆菌显示出最大的敏感性，而野油菜黄单胞菌则表现出抗性。
