口腔黏膜灶性上皮增生中13型和32型人乳头瘤病毒的原位检测
In situ detection of human papillomavirus types 13 and 32 in focal epithelial hyperplasia of the oral mucosa.
作者信息
Henke R P, Guèrin-Reverchon I, Milde-Langosch K, Koppang H S, Löning T
机构信息
Institute of Pathology, University of Hamburg, West Germany.
出版信息
J Oral Pathol Med. 1989 Aug;18(7):419-21. doi: 10.1111/j.1600-0714.1989.tb01575.x.
17 cases of focal epithelial hyperplasia of the oral mucosa (FEH, Heck's disease) were investigated for the presence of human papillomavirus (HPV) nucleic acid sequences by means of in situ DNA hybridization using biotinylated DNA probes of HPV types 1, 6, 11, 13, 16, 18, and 32. Ten of 17 cases were positive for HPV 13 DNA in contrast to 6 of 17 positive cases obtained after application of the HPV 32 probe, with a double infection in one case. The results of our study suggest, that HPV 13 and HPV 32 are very specifically found in lesions of FEH and can be detected in a high percentage of cases using in situ hybridization.
采用生物素化的1型、6型、11型、13型、16型、18型和32型人乳头瘤病毒（HPV）DNA探针，通过原位DNA杂交法，对17例口腔黏膜局灶性上皮增生（FEH，赫克病）患者进行HPV核酸序列检测。17例患者中，10例HPV 13 DNA呈阳性；相比之下，应用HPV 32探针后，17例中有6例呈阳性，其中1例为双重感染。我们的研究结果表明，HPV 13和HPV 32在FEH病变中具有高度特异性，采用原位杂交法可在高比例病例中检测到。
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