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猪滑膜胶原酶的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of porcine synovial collagenase.

作者信息

Lloyd L F, Skarzyński T, Wonacott A J, Cawston T E, Clark I M, Mannix C J, Harper G P

机构信息

Blackett Laboratory, Imperial College, London, U.K.

出版信息

J Mol Biol. 1989 Nov 5;210(1):237-8. doi: 10.1016/0022-2836(89)90304-5.

Abstract

Crystals of porcine synovial collagenase suitable for an X-ray structure analysis have been obtained. The crystals belong to space group I4, with unit cell dimensions a = b = 160.0 A, c = 53.1 A, with one molecule in the asymmetric unit. Diffraction extends beyond 3 A perpendicular to the c axis but along the 4-fold axis, the intensities are measurable only to 4 A.

摘要

已获得适合进行X射线结构分析的猪滑膜胶原酶晶体。这些晶体属于空间群I4,晶胞参数为a = b = 160.0 Å,c = 53.1 Å,不对称单元中有一个分子。垂直于c轴方向衍射延伸至3 Å以外,但沿四重轴方向,强度仅在4 Å处可测量。

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