Numazaki K, Goldman H, Bai X Q, Wong I, Wainberg M A
Montreal Children's Hospital Research Institute, Quebec, Canada.
Microbiol Immunol. 1989;33(9):733-45. doi: 10.1111/j.1348-0421.1989.tb00960.x.
A tissue culture system for the growth of human fetal and infantile thymic epithelial (TE) cells has been established and characterized. We have investigated the effects of infection of these cells by human cytomegalovirus (CMV), measles virus, and human immunodeficiency virus type-1 (HIV-1). In the case of CMV, morphological changes were apparent by 2-4 days after viral inoculation of infantile TE cells. CMV-related antigens were detected by immunofluorescence after 12 days, and progeny infectious CMV was recovered from culture media after 18 days. Following infection by measles virus, distinctive, multinucleated giant TE cells appeared in both cultures of fetal and infantile TE cells. Measles virus-inoculated TE cells displayed an altered phenotype, as revealed by reaction with monoclonal antibodies with specificity for a variety of TE markers. Finally, infection of TE cells by HIV-1 resulted in cellular disarrangement, increased numbers of Hassall's corpuscles, and multinucleated giant cells. An increase in the number of cells reactive with monoclonal antibodies, specific for Hassall's corpuscles, was observed in the case of cells infected by either measles virus or HIV-1. These findings suggest that a variety of different viruses can successfully infect thymic epithelial tissue. Because of the important role of the thymus in development of the immune system, it is reasonable to conclude that viral infection of thymic tissue might play an important role in virus-mediated suppression of immune responsiveness.
一种用于人类胎儿和婴儿胸腺上皮（TE）细胞生长的组织培养系统已建立并得到表征。我们研究了人巨细胞病毒（CMV）、麻疹病毒和1型人类免疫缺陷病毒（HIV-1）对这些细胞的感染作用。就CMV而言，在将病毒接种到婴儿TE细胞后2 - 4天，形态学变化就很明显。12天后通过免疫荧光检测到CMV相关抗原，18天后从培养基中回收了有传染性的CMV子代。在麻疹病毒感染后，胎儿和婴儿TE细胞培养物中均出现了独特的多核巨TE细胞。接种麻疹病毒的TE细胞表现出改变的表型，这通过与针对多种TE标志物具有特异性的单克隆抗体反应得以揭示。最后，HIV-1感染TE细胞导致细胞排列紊乱、哈氏小体数量增加以及多核巨细胞出现。在麻疹病毒或HIV-1感染的细胞中，观察到与针对哈氏小体具有特异性的单克隆抗体反应的细胞数量增加。这些发现表明多种不同病毒可成功感染胸腺上皮组织。由于胸腺在免疫系统发育中起重要作用，合理的结论是胸腺组织的病毒感染可能在病毒介导的免疫反应抑制中起重要作用。
