Wainberg M A, Numazaki K, Destephano L, Wong I, Goldman H
Department of Microbiology, Montreal Children's Hospital and Research Institute, Quebec, Canada.
Clin Exp Immunol. 1988 Jun;72(3):415-21.
Cultures of human thymic epithelial (TE) cells are able to produce a interleukin 1 (IL-1) like activity. This IL-1 activity can be detected either using mouse thymocytes in a traditional IL-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. Production of IL-1 activity by TE cells was found to be maximal between 3 and 4 weeks after culture initiation. Human thymocytes worked best as targets in an IL-1 assay, when these cells were derived from donors younger than 1 year of age. Infection of human TE cells by any of human cytomegalovirus, herpes simplex virus type 2, adenovirus 7, Coxsackie B1, and respiratory syncytial virus led to marked reductions in the ability of these cells to secrete measurable IL-1 activity. In the case of TE cells infected by cytomegalovirus, respiratory syncytial virus, and Coxsackie B1, this abrogation of production of IL-1 activity occurred in the absence of any obvious virus-induced cytopathic effect.
人胸腺上皮（TE）细胞培养物能够产生一种白细胞介素1（IL-1）样活性。这种IL-1活性可以通过在传统的IL-1检测中使用小鼠胸腺细胞，或者使用从儿科心血管手术病例中获得的胸腺淋巴细胞来检测。发现TE细胞产生IL-1活性在培养开始后3至4周时最高。当人胸腺细胞来自1岁以下的供体时，它们在IL-1检测中作为靶细胞效果最佳。人巨细胞病毒、单纯疱疹病毒2型、腺病毒7型、柯萨奇B1病毒和呼吸道合胞病毒中的任何一种感染人TE细胞，都会导致这些细胞分泌可测量的IL-1活性的能力显著降低。在被巨细胞病毒、呼吸道合胞病毒和柯萨奇B1病毒感染的TE细胞中，IL-1活性产生的这种消除在没有任何明显的病毒诱导细胞病变效应的情况下发生。
