Numazaki K, Bai X Q, Goldman H, Wong I, Spira B, Wainberg M A
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Clin Immunol Immunopathol. 1989 May;51(2):185-95. doi: 10.1016/0090-1229(89)90018-4.
We have succeeded in growing the HTLV-IIIB strain of human immunodeficiency virus type 1 (HIV-1) in cultured human thymic epithelial (TE) cells. Expression of the HIV-1 proteins p17 and p24 was detected by immunofluorescence and reached a peak at 3 days after infection. Antigen capture and reverse transcriptase assays were used to detect HIV-1 in culture fluids, with positive results also being realized. The infection was cytolytic; cellular disarrangement, increased numbers of Hassall's corpuscles, and giant cells first appeared in monolayers of TE cells at 2 days after inoculation. By 4 days these changes were increased, and by 7 days, retraction and involution of TE cells were evident. The infection of TE cells by HIV-1 was blocked by preincubation with monoclonal OKT4A antibodies directed against CD4 target molecules.
我们成功地在培养的人胸腺上皮（TE）细胞中培养出了1型人类免疫缺陷病毒（HIV-1）的HTLV-IIIB毒株。通过免疫荧光检测到HIV-1蛋白p17和p24的表达，并且在感染后3天达到峰值。采用抗原捕获和逆转录酶测定法检测培养液中的HIV-1，也得到了阳性结果。该感染具有细胞溶解性；细胞排列紊乱、哈氏小体数量增加以及巨细胞在接种后2天首次出现在TE细胞单层中。到4天时这些变化加剧，到7天时，TE细胞的回缩和退化明显可见。用针对CD4靶分子的单克隆OKT4A抗体进行预孵育可阻断HIV-1对TE细胞的感染。
