Ellard F M, Cabello A, Salmond G P
Department of Biological Sciences, University of Warwick, Coventry, UK.
Mol Gen Genet. 1989 Sep;218(3):491-8. doi: 10.1007/BF00332415.
Using transformation and conjugal mobilization, plasmids carrying the lamB gene of Escherichia coli were transferred to a range of Erwinia strains. The resultant strains were infected with lambda 467, and kanamycin resistant transductants were screened for various mutant phenotypes including auxotrophy and altered extracellular enzyme activities. Reversion analysis suggested that most mutant phenotypes were due to Tn5 insertion. The applicability of the techniques was highly strain dependent. However a rapid and simple route to mutant isolation was obtained, which could allow the use of other lambda-related genetic techniques in several important species which, to date, have not been genetically manipulated.
利用转化和接合转移，将携带大肠杆菌lamB基因的质粒转移到一系列欧文氏菌菌株中。用λ467感染所得菌株，并筛选卡那霉素抗性转导子的各种突变表型，包括营养缺陷型和细胞外酶活性改变。回复分析表明，大多数突变表型是由于Tn5插入所致。这些技术的适用性高度依赖于菌株。然而，获得了一条快速简单的突变体分离途径，这使得在一些迄今为止尚未进行基因操作的重要物种中能够使用其他与λ相关的遗传技术。
