Chung Wen Yuan, Gravante Gianpiero, Eltweri Amar, Sorge Roberto, Ong Seok Ling, Pollard Cristina, Metcalfe Mathew, Dennison Ashley
Department of Hepatobiliary and Pancreatic Surgery, Leicester General Hospital, University of Leicester, Gwendolen Road, Leicester, LE5 4PW, UK,
J Artif Organs. 2015 Jun;18(2):151-61. doi: 10.1007/s10047-014-0813-x. Epub 2015 Jan 4.
The multiorgan ex vivo perfused liver-kidney model allows studying the hepatic pathophysiology and purifying waste products. We tested if the addition of the kidney first followed by the liver (KL circuit) produces better results compared to the classic liver-first approach (LK). Intact livers and kidneys were obtained post mortem from ten female domestic white pigs, five experiments were conducted with the KL circuit and five with the LK. Bile, urine production, arterial blood gases, glucose, renal and liver tests were collected hourly during the perfusions. The KL circuit had values more close to physiological ranges, more stable over time and showed less variability compared to the LK circuit for urine production, glucose, PH, anion gap, lactate, urea, sodium, potassium and Alanine Transaminase (ANOVA test for repeated measures p < 0.05). The KL circuit produced a more physiological and reliable biochemical milieu.
多器官体外灌注肝肾模型可用于研究肝脏病理生理学和净化废物。我们测试了先连接肾脏再连接肝脏的回路（KL回路）与传统的先连接肝脏的方法（LK）相比是否能产生更好的结果。从10只雌性家养白猪死后获取完整的肝脏和肾脏，使用KL回路进行了5次实验，使用LK进行了5次实验。在灌注过程中每小时收集胆汁、尿液生成量、动脉血气、葡萄糖、肾脏和肝脏检测指标。与LK回路相比，KL回路在尿液生成量、葡萄糖、pH值、阴离子间隙、乳酸、尿素、钠、钾和丙氨酸转氨酶方面的值更接近生理范围，随时间更稳定，且变异性更小（重复测量方差分析，p < 0.05）。KL回路产生了更符合生理且更可靠的生化环境。
