Properties of excitatory amino acid receptors on sustained ganglion cells in the cat retina.

Iontophoretic effects of N-methyl-D-aspartate, quisqualate and kainate and a variety of excitatory amino acid receptor antagonists, on retinal ganglion cells, were studied in optically intact eyes of barbiturate anesthetized cats. All three agonists raised the spontaneous firing of both ON- and OFF-sustained retinal ganglion cells, with the potency order of kainate much greater than quisqualate greater than N-methyl-D-aspartate. However, the excitatory amino acid analogues readily saturated the receptors and reduced the visually driven firing of cells with high spontaneous firing, but mimicked an increase in endogenous excitatory amino acid release and raised the visually induced response in cells with low spontaneous firing. The quinoxaline compound, 6-cyano-2,3 dihydroxy-7-nitroquinoxaline and 6-7-dinitroquinoxaline-2,3-dione, blocked the visually driven firing and kainate- and quisqualate-induced excitation, whilst 3[+)-2-carboxypiperazin-4-yl)propyl-1-phosphonate, antagonized the N-methyl-D-aspartate-induced excitation, but failed to block visually driven firing of the retinal ganglion cells. The broadband excitatory amino acid receptor antagonists, such as kynurenate, were also effective in antagonizing the visually driven response and also blocked the N-methyl-D-aspartate- as well as kainate- and quisqualate-induced responses. These results suggest that the receptors at the bipolar/ganglion cell synapse are of the non-N-methyl-D-aspartate type, but that N-methyl-D-aspartate receptors are also present on ganglion cells although their physiological role is unclear.