Aydın A F, Çoban J, Doğan-Ekici I, Doğru-Abbasoğlu S, Uysal M, Koçak-Toker N
Department of Biochemistry, İstanbul Medical Faculty, Istanbul University, Çapa, İstanbul, Turkey.
Department of Biochemistry, Yeditepe University Medical Faculty, Kayışdağı, İstanbul, Turkey.
Andrologia. 2015 Dec;47(10):1131-8. doi: 10.1111/and.12392. Epub 2014 Dec 29.
Oxidative stress is considered to play a key role in ageing. Carnosine alone or together with vitamin E may prove to be helpful in dealing with problems of ageing through its antioxidant activity. Testis, by producing steroids and possessing a poor antioxidant system may become a strong target for the chronic oxidative stress generated during ageing. Therefore we investigated the in vivo effect of carnosine alone or together with vitamin E on testicular oxidative stress in aged rats. In this study, young (5 months) and aged (22 months) Wistar rats were used. Carnosine (250 mg kg(-1); i.p.; 5 days per week) and vitamin E (200 mg kg(-1); i.m.; twice per week) were given to aged rats for 2 months. Increased testicular lipid peroxidation and superoxide dismutase activity in aged rats were declined to the levels of young ones by both treatments. Decreased glutathione peroxidase and glutathione transferase activities returned to the level of young's only by carnosine plus vitamin E treatment. Histopathological evaluation described by Johnsen's score, also showed significant improvement with preserved spermatogenesis. Carnosine plus vitamin E treatment appears to stage a powerful performance by attenuating testicular oxidative stress and sparing the antioxidant system.
氧化应激被认为在衰老过程中起关键作用。肌肽单独或与维生素E一起，可能因其抗氧化活性而有助于应对衰老问题。睾丸通过产生类固醇且拥有较差的抗氧化系统，可能成为衰老过程中产生的慢性氧化应激的强大靶点。因此，我们研究了肌肽单独或与维生素E一起对老年大鼠睾丸氧化应激的体内作用。在本研究中，使用了年轻（5个月）和老年（22个月）的Wistar大鼠。给老年大鼠注射肌肽（250 mg kg(-1)；腹腔注射；每周5天）和维生素E（200 mg kg(-1)；肌肉注射；每周两次），持续2个月。两种处理均使老年大鼠睾丸脂质过氧化增加和超氧化物歧化酶活性下降至年轻大鼠的水平。谷胱甘肽过氧化物酶和谷胱甘肽转移酶活性降低，仅通过肌肽加维生素E处理恢复到年轻大鼠的水平。用约翰森评分法进行的组织病理学评估也显示，精子发生得以保留，有显著改善。肌肽加维生素E处理似乎通过减轻睾丸氧化应激和保护抗氧化系统而发挥强大作用。
